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FlyAtlas: the Drosophila gene expression atlas

University of Glasgow
Biotechnology & Biological Sciences Research Council
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Latest news: FlyAtlas2 : A Database web-app for fruitfly expression with added RNA & miRNA goodness ! Click here to visit the application. Read all about it here.

This dataset was generated by Venkat Chintapalli, Jing Wang & Julian Dow at the University of Glasgow with funding from the UK's BBSRC.
It gives you a quick answer to the question: where is my gene of interest expressed/enriched in the adult fly?
For each gene & tissue, you're given the mRNA SIGNAL (how abundant the mRNA is), the mRNA ENRICHMENT (compared to whole flies), and the Affymetrix PRESENT CALL (out of 4 arrays, how many times it was detectably expressed).

String to look for? (e.g. vha, cell adhesion, receptor, aquaporin, adenylate, CG1147, pnt)

Searching for FBGN0032464 through 18771 annotations produced 1 hits

Vacuolar H[+] ATPase subunit 68-3 (Vha68-3):

Annotation Number : CG5075
FlyAtlas2 : Click Here for expression data based on RNA-Seq and or miRNA-Seq analyses
Flybase ID : FBgn0032464
Gbrowse : chr2L:12978785-12981301 (+) // 100.0 //
Probeset : 1628908_at
Accessions : protein= NP_609595;, nucleotide= NM_135751;
Pathway : "0000221 // vacuolar proton-transporting V-type ATPase
Interpro : V1 domain // inferred from sequence or structural similarity /// 0016469 // proton-transporting two-sector ATPase compl
FlyMine data for FBgn0032464.
Homophila reports that CG5075 is a close match (1e-17) to human gene ATP6B1;VPP3 (NP_001683), a gene associated with Renal tubular acidosis with deafness, 267300 (3).
VDRC has 3 RNAi stocks for FBgn0032464 available for purchase.

TissuemRNA SignalPresent CallEnrichmentAffy Call
Brain1 00 of 4 0.00Down
Head0 00 of 4 0.00Down
Eye0 00 of 4 0.01Down
Thoracicoabdominal ganglion2 10 of 4 0.00Down
Salivary gland10 30 of 4 0.09Down
Crop5 21 of 4 0.10Down
Midgut2 10 of 4 0.00Down
Tubule3 00 of 4 0.00Down
Hindgut3 00 of 4 0.00Down
Heart2 10 of 4 0.02Down
Fat body0 00 of 4 0.01Down
Ovary1 00 of 4 0.00Down
Testis1403 394 of 412.80Up
Male accessory glands3 00 of 4 0.00Down
Virgin spermatheca3 10 of 4 0.03Down
Mated spermatheca3 10 of 4 0.03Down
Adult carcass11 41 of 4 0.10Down
Larval CNS2 00 of 4 0.02Down
Larval Salivary gland4 10 of 4 0.04Down
Larval midgut6 01 of 4 0.06Down
Larval tubule1 00 of 4 0.00Down
Larval hindgut1 00 of 4 0.01Down
Larval fat body145 824 of 4 1.30None
Larval trachea3 51 of 4 0.03Down
Larval carcass2 10 of 4 0.02Down
S2 cells (growing)3 10 of 4 0.03Down
Whole fly109 54 of 4

Looking for FBgn0032464. 1 entries matched from 18771, in 2 s.

Chintapalli, V. R., Wang, J. and Dow, J. A. T. (2007). Using FlyAtlas to identify better Drosophila models of human disease. Nature Genetics 39: 715-720

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