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FlyAtlas: the Drosophila gene expression atlas

University of Glasgow
Biotechnology & Biological Sciences Research Council
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Latest news: FlyAtlas2 : A Database web-app for fruitfly expression with added RNA & miRNA goodness ! Click here to visit the application. Read all about it here.

This dataset was generated by Venkat Chintapalli, Jing Wang & Julian Dow at the University of Glasgow with funding from the UK's BBSRC.
It gives you a quick answer to the question: where is my gene of interest expressed/enriched in the adult fly?
For each gene & tissue, you're given the mRNA SIGNAL (how abundant the mRNA is), the mRNA ENRICHMENT (compared to whole flies), and the Affymetrix PRESENT CALL (out of 4 arrays, how many times it was detectably expressed).

String to look for? (e.g. vha, cell adhesion, receptor, aquaporin, adenylate, CG1147, pnt)

Searching for FBGN0040705 through 18771 annotations produced 1 hits

NADH ubiquinone oxidoreductase B8 (CG15434):

Annotation Number : CG15434
FlyAtlas2 : Click Here for expression data based on RNA-Seq and or miRNA-Seq analyses
Flybase ID : FBgn0040705
Gbrowse : chr2L:4454664-4455160 (+) // 100.0 //
Probeset : 1640052_at
Accessions : protein= NP_652341;, nucleotide= NM_144084;
Pathway : NADH to ubiquinone // inferred from sequence or structural similarity /// 0055114 // oxidation reduction // inferred fr
Interpro : 0005747 // mitochondrial respiratory chain complex I // inferred from sequence or structural similarity
FlyMine data for FBgn0040705.
Homophila reports that CG15434 is a close match (1e-17) to human gene NDUFA2 (NP_002479), a gene associated with Leigh syndrome due to mitochondrial complex I deficiency, 256000 (3).
VDRC has 2 RNAi stocks for FBgn0040705 available for purchase.

TissuemRNA SignalPresent CallEnrichmentAffy Call
Brain1 00 of 4 0.00Down
Head3 10 of 4 0.00Down
Eye4 10 of 4 0.06Down
Thoracicoabdominal ganglion2 00 of 4 0.00Down
Salivary gland1 00 of 4 0.02Down
Crop2 10 of 4 0.00Down
Midgut2 10 of 4 0.00Down
Tubule1 00 of 4 0.00Down
Hindgut0 00 of 4 0.00Down
Heart7 40 of 4 0.10Down
Fat body4 40 of 4 0.06Down
Ovary1 00 of 4 0.00Down
Testis157 134 of 4 1.90Up
Male accessory glands3 10 of 4 0.00Down
Virgin spermatheca1 00 of 4 0.01Down
Mated spermatheca0 00 of 4 0.01Down
Adult carcass288 154 of 4 3.50Up
Larval CNS2 10 of 4 0.03Down
Larval Salivary gland1 00 of 4 0.02Down
Larval midgut2 10 of 4 0.03Down
Larval tubule1 00 of 4 0.00Down
Larval hindgut0 00 of 4 0.01Down
Larval fat body7 41 of 4 0.10Down
Larval trachea2 40 of 4 0.03Down
Larval carcass2 00 of 4 0.04Down
S2 cells (growing)1 00 of 4 0.02Down
Whole fly82 214 of 4

Looking for FBgn0040705. 1 entries matched from 18771, in 2 s.

Chintapalli, V. R., Wang, J. and Dow, J. A. T. (2007). Using FlyAtlas to identify better Drosophila models of human disease. Nature Genetics 39: 715-720

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