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FlyAtlas: the Drosophila gene expression atlas

University of Glasgow
Biotechnology & Biological Sciences Research Council
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Latest news: FlyAtlas2 : A Database web-app for fruitfly expression with added RNA & miRNA goodness ! Click here to visit the application. Read all about it here.


This dataset was generated by Venkat Chintapalli, Jing Wang & Julian Dow at the University of Glasgow with funding from the UK's BBSRC.
It gives you a quick answer to the question: where is my gene of interest expressed/enriched in the adult fly?
For each gene & tissue, you're given the mRNA SIGNAL (how abundant the mRNA is), the mRNA ENRICHMENT (compared to whole flies), and the Affymetrix PRESENT CALL (out of 4 arrays, how many times it was detectably expressed).

looking for 1628427_a_at:

>probe:Drosophila_2:1628427_a_at:229:289; Interrogation_Position=1003; Antisense; CGGAATAGCTGTGCGATCGCGTGAT
>probe:Drosophila_2:1628427_a_at:308:45; Interrogation_Position=1018; Antisense; ATCGCGTGATCGTTGCCTTGTAGGC
>probe:Drosophila_2:1628427_a_at:97:271; Interrogation_Position=1081; Antisense; CATTAACTTTAATTGAGCTCTACTT
>probe:Drosophila_2:1628427_a_at:396:417; Interrogation_Position=1095; Antisense; GAGCTCTACTTATGTACTCTAGGCT
>probe:Drosophila_2:1628427_a_at:409:145; Interrogation_Position=1110; Antisense; ACTCTAGGCTATAACACACACACAC
>probe:Drosophila_2:1628427_a_at:614:671; Interrogation_Position=1159; Antisense; TACCCATTACCATACACACATTCAA
>probe:Drosophila_2:1628427_a_at:107:157; Interrogation_Position=1174; Antisense; ACACATTCAACCCAGCTCTTTGGTT
>probe:Drosophila_2:1628427_a_at:385:309; Interrogation_Position=1185; Antisense; CCAGCTCTTTGGTTATATGTTTTGT
>probe:Drosophila_2:1628427_a_at:131:495; Interrogation_Position=1232; Antisense; GTCAACAATGTTTACTTTTTACACA
>probe:Drosophila_2:1628427_a_at:600:489; Interrogation_Position=1257; Antisense; GTAAAGCATGCACCTTTTTTAGTTT
>probe:Drosophila_2:1628427_a_at:222:303; Interrogation_Position=1309; Antisense; CCCCAACTTCTCTATTTTGCATTAA
>probe:Drosophila_2:1628427_a_at:504:617; Interrogation_Position=1380; Antisense; TGCATCAAACACATCAACATATAAG
>probe:Drosophila_2:1628427_a_at:221:325; Interrogation_Position=1536; Antisense; GCGAATTCAGCAAGAGAAGCACACA
>probe:Drosophila_2:1628427_a_at:376:377; Interrogation_Position=1551; Antisense; GAAGCACACACAAAGGCGAATTGAT

Paste this into a BLAST search page for me
CGGAATAGCTGTGCGATCGCGTGATATCGCGTGATCGTTGCCTTGTAGGCCATTAACTTTAATTGAGCTCTACTTGAGCTCTACTTATGTACTCTAGGCTACTCTAGGCTATAACACACACACACTACCCATTACCATACACACATTCAAACACATTCAACCCAGCTCTTTGGTTCCAGCTCTTTGGTTATATGTTTTGTGTCAACAATGTTTACTTTTTACACAGTAAAGCATGCACCTTTTTTAGTTTCCCCAACTTCTCTATTTTGCATTAATGCATCAAACACATCAACATATAAGGCGAATTCAGCAAGAGAAGCACACAGAAGCACACACAAAGGCGAATTGAT

Full Affymetrix probeset data:

Annotations for 1628427_a_at in Drosophila_2.na32.annot.csv


Chintapalli, V. R., Wang, J. and Dow, J. A. T. (2007). Using FlyAtlas to identify better Drosophila models of human disease. Nature Genetics 39: 715-720

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