FlyAtlas: the Drosophila gene expression atlas

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Latest news: FlyAtlas2 : A Database web-app for fruitfly expression with added RNA & miRNA goodness ! Click here to visit the application. Read all about it here.

This dataset was generated by Venkat Chintapalli, Jing Wang & Julian Dow at the University of Glasgow with funding from the UK's BBSRC.
It gives you a quick answer to the question: where is my gene of interest expressed/enriched in the adult fly?
For each gene & tissue, you're given the mRNA SIGNAL (how abundant the mRNA is), the mRNA ENRICHMENT (compared to whole flies), and the Affymetrix PRESENT CALL (out of 4 arrays, how many times it was detectably expressed).

looking for 1635306_at:

>probe:Drosophila_2:1635306_at:111:341; Interrogation_Position=349; Antisense; GCTATTCTCGGCTTGGCTACGGATA
>probe:Drosophila_2:1635306_at:625:571; Interrogation_Position=363; Antisense; GGCTACGGATATTGTGTTTTCGAAA
>probe:Drosophila_2:1635306_at:67:247; Interrogation_Position=398; Antisense; CAATTTGCATAGTGTGGTGGACCAT
>probe:Drosophila_2:1635306_at:426:29; Interrogation_Position=445; Antisense; ATACAGGTGCTAAGCGGTGCTCAAT
>probe:Drosophila_2:1635306_at:460:531; Interrogation_Position=471; Antisense; GGGTCTACCCAATGATCGAAATGAA
>probe:Drosophila_2:1635306_at:95:23; Interrogation_Position=539; Antisense; ATATGTGTTCTACCCTAAACGGCAC
>probe:Drosophila_2:1635306_at:660:265; Interrogation_Position=580; Antisense; CAGTTTTGTGCTGGAGACTCGGATT
>probe:Drosophila_2:1635306_at:61:725; Interrogation_Position=620; Antisense; TTGACTTTAGCAGTCCTCTGGGAGC
>probe:Drosophila_2:1635306_at:314:641; Interrogation_Position=636; Antisense; TCTGGGAGCCATTATAACCTTCAAG
>probe:Drosophila_2:1635306_at:134:13; Interrogation_Position=664; Antisense; ATTCAGCGCTATGTCCTAATTGGCA
>probe:Drosophila_2:1635306_at:212:361; Interrogation_Position=718; Antisense; GCAAGCGTTTATACGGATGTCCTGA
>probe:Drosophila_2:1635306_at:712:441; Interrogation_Position=733; Antisense; GATGTCCTGAGTCACACCGATTTTA
>probe:Drosophila_2:1635306_at:480:155; Interrogation_Position=746; Antisense; ACACCGATTTTATTCTTTCAGTATG
>probe:Drosophila_2:1635306_at:469:681; Interrogation_Position=767; Antisense; TATGGCGACAGTACCGCAACGGTGA

Paste this into a BLAST search page for me
GCTATTCTCGGCTTGGCTACGGATAGGCTACGGATATTGTGTTTTCGAAACAATTTGCATAGTGTGGTGGACCATATACAGGTGCTAAGCGGTGCTCAATGGGTCTACCCAATGATCGAAATGAAATATGTGTTCTACCCTAAACGGCACCAGTTTTGTGCTGGAGACTCGGATTTTGACTTTAGCAGTCCTCTGGGAGCTCTGGGAGCCATTATAACCTTCAAGATTCAGCGCTATGTCCTAATTGGCAGCAAGCGTTTATACGGATGTCCTGAGATGTCCTGAGTCACACCGATTTTAACACCGATTTTATTCTTTCAGTATGTATGGCGACAGTACCGCAACGGTGA

Full Affymetrix probeset data:

Annotations for 1635306_at in Drosophila_2.na32.annot.csv

Chintapalli, V. R., Wang, J. and Dow, J. A. T. (2007). Using FlyAtlas to identify better Drosophila models of human disease. Nature Genetics 39: 715-720